The introduction of an entrepreneurship education makerspace at Rowan University supports the evolution of a cross-campus and interdisciplinary culture of innovation and entrepreneurship. Developed as an extension of the Rowan Center for Innovation and Entrepreneurship, the Studio 231 makerspace supports core, co-curricular and extracurricular efforts in design, prototyping, and entrepreneurship education, promoting an entrepreneurial mindset throughout the University. Configured as an experiential learning lab to demonstrate, teach, and conduct low- and high-resolution prototyping, Studio 231 has become a communal workspace to provoke and accelerate entrepreneurial collaborations among students and faculty from the University’s 12 colleges and schools. Outfitted with a diverse set of tools and materials, including staff delivering experiential learning instructional modules and themed sessions, the makerspace enables entrepreneurs to turn their ideas into reality.