The purpose of this chapter is to support entrepreneurship scholars in their efforts to integrate neuroimaging with entrepreneurship. First, we describe the potential benefits of using neuroimaging in entrepreneurship research. Second, we discuss general issues and considerations to make in neuroimaging research. Then we focus on functional magnetic resonance imaging (fMRI) as a neuroimaging method to bring the neural level of analysis to entrepreneurship research. We discuss opportunity recognition and assessment, and entrepreneurial decision making as examples of topic areas ripe for neuroentrepreneurship and fMRI. We subsequently describe hypotheses designs and best practices in fMRI research. Finally, we close with a discussion regarding interpretation and communication of results.